Isolation of Frankia Strains from Alder Actinorhizal Root Nodules
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چکیده
منابع مشابه
Isolation of frankia strains from alder actinorhizal root nodules.
A simple procedure, based on the rapid filtration and washing of Frankia vesicle clusters, was devised for the isolation of Frankia strains from alder actinorhizal root nodules. Of 46 Alnus incana subsp. rugosa nodules prepared, 42 yielded isolates. A simple medium containing mineral salts, Casamino Acids, and sodium pyruvate proved to be the most effective for isolation. In general, colonies a...
متن کاملNatural diversity of Frankia strains in actinorhizal root nodules from promiscuous hosts in the family Myricaceae.
Actinorhizal plants invade nitrogen-poor soils because of their ability to form root nodule symbioses with N(2)-fixing actinomycetes known as Frankia. Frankia strains are difficult to isolate, so the diversity of strains inhabiting nodules in nature is not known. To address this problem, we have used the variability in bacterial 16S rRNA gene sequences amplified from root nodules as a means to ...
متن کاملSymbiosis between Frankia and actinorhizal plants: root nodules of non-legumes.
In actinorhizal symbioses, filamentous nitrogen-fixing soil bacteria of the genus Frankia induce the formation of nodules on the roots of a diverse group of dicotyledonous plants representing trees or woody shrubs, with one exception, Datisca glomerata. In the nodules, Frankia fixes nitrogen and exports the products to the plant cytoplasm, while being supplied with carbon sources by the host. P...
متن کاملDiversity of frankia strains in root nodules of plants from the families elaeagnaceae and rhamnaceae
Partial 16S ribosomal DNAs (rDNAs) were PCR amplified and sequenced from Frankia strains living in root nodules of plants belonging to the families Elaeagnaceae and Rhamnaceae, including Colletia hystrix, Elaeagnus angustifolia, an unidentified Elaeagnus sp., Talguenea quinquenervia, and Trevoa trinervis. Nearly full-length 16S rDNAs were sequenced from strains of Frankia living in nodules of C...
متن کاملThe isolation of Frankia from nodules of Casuarina
DIEM, H. G., and Y. DOMMERGUES. 1983. The isolation of Frankia from nodules of Casuarina. Can. J. Bot. 61: 2822-2825. Attempting to isolate Frankia from Casuarina, we found that this endophyte exhibited two unexpected characteristics: ( i ) an inability of most infected nodule cells to produce Frankia colonies; and ( i i ) the absence of specific nutritional requirements in the kOht iO! l mCdi?...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1982
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.44.2.461-465.1982